News

Tumor cell and mutational landscape in colorectal cancer development

We are very glad to inform you that the Laboratory of Translational Cancer Genomics will start new project awarded by Czech Health Research Council. The project will be carried out in cooperation with the University Hospital in Pilsen and Institute of Experimental Medicine Czech Academy of Sciences.


Telomere homeostasis as potential biomarker of risk, prognosis and progression in gynaecologic tumours

We are very glad to inform you that the Laboratory of Translational Cancer Genomics will collaborate with  Institute of Experimental Medicine Czech Academy of Sciences and Thomayer hospital on the new project awarded by the Czech Health Research Council.


CHROMOSOMAL ABERRATION IN HEALTHY PERSONS

Blood white cells of healthy individuals may contain non-recurrent, random chromosomal aberrations (CAs), which disappear with cell turnover as they provide no growth advantage. These include chromatid breaks, fragmented or missing parts of chromosomes and fusions resulting in dicentric and ring chromosomes. These nonspecific CAs have been used in monitoring of occupational populations exposed to potential carcinogenic chemicals and radiation. The analysis of CA proceeds in many steps (Figure). The specimen in the figure is the fresh blood sample, which is cultured and harvested for slide preparation; the slides are then analyzed to score CAs.


Project Management Course

European Center Charles University invites you to the Project Management Course which will be held on 19 April 2021.Invitation here.


T-cells as main actors in anti-tumor immunity

The long-suspected role of the importance of immunity in cancer has gained strong experimental support in the past decade. Infiltrating immune cells can function to control tumor growth or to help create an immunosuppressive environment in which the tumor can thrive. Immune surveillance by T-cells in human cancers is one of the key mechanisms of anti-cancer immunity.


Ph.D. Positions at the Biomedical Center

Students for the following Ph.D. study topics are sought in the doctoral study programme:


PROPER Course

Are you a Ph.D. student at the Faculty of medicine in Pilsen and do you want to better understand the basic principles of study design, critical appraisal, research governance and medical writing in experimental research? Register for an online course Protocol, Reviewing, Ownership and Publishing in Experimental Research“ (PROPER) by Prof. Khaled Ismail. Four modules of the course will take place from March till June, 2021.


Assessment of tumor cellular microenvironment

Hepatocellular carcinoma (HCC) occurs in people with an underlying liver disease of either viral or toxic origin. It is usually preceded by cirrhosis. In the Czech Republic alcoholic liver disease is the major etiological factor. Tumor microenvironment is composed of myeloid (innate immunity) and lymphoid (adaptive immunity) lineages. Infiltrating immune cells can function to control tumor growth or to help create an immunosuppressive environment in which the tumor can thrive. The long suspected role for immune surveillance in human cancer has gained strong experimental support in the past decade. The concepts of ‘hot’ or ‘cold’ tumors refer to signs of inflammation (hot) when the tumor has already been infiltrated by T cells, which usually correlates with a response to immunotherapy. Even more gratifying, these scientific breakthroughs have also benefited clinical oncology in the context of diagnostics, prognostication, drug development and therapy.


Telomere length and its role in cancer

Normally human cells can divide only a few dozen of times and they can enter senescence. In contrary, the number of cancer cell divisions is not limited and hence they seem immortal. It turned out this limitation is imposed by length of the so called telomeres.


TERT promoter mutations in skin disease before and after treatment

Squamous cell carcinoma is a common skin cancer. It does not usually develop metastases and can be cured by surgery. However, as exposure to solar irradiation is the major risk factor for this skin cancer, the location of tumors are at sun-exposed sites, usually on the face. In this study we collaborated with Finnish dermatologists who treated patients with actinic keratosis, the precursor of squamous cell skin cancer.

 

 


Open science: from data to publication

Open Science Support Centre invites you to the Seminar Open science: from data to publication. For more information click here


Chaperon team has a new personnel acquisition

On December 1, Andriy Trailin started in the team on Senior Researcher position. His research will be focused on pathological and immunohistochemical part of the project.


TERT promoter mutations in cancer

The Laboratory of Translational Cancer Genomics together with Laboratory of Pharmacogenomics are presently working on TERT promoter mutation profiling in hepatocellular carcinoma and adjacent non tumour tissue.


Single-cell analysis in cancer research

In September 2020, a single-cell training course with a dense programme was organized by Dr. Monika Holubová from a neighboring lab in the Biomedical Center. The course was open to applicants from all European countries. The course started with introductory lectures about isolation of individual cells, approaches to perform PCR reactions in a large scale using microfluidics devices, how to prepare sequencing libraries and perform their quality control. The following days were aimed at hands-on lab work.


Melanoma – a dangerous skin cancer

Melanoma is a potentially fatal skin disease for which sun exposure is an important risk factor. For this reason people with red or blond hair, blue or green eyes, and fair skin that freckles or burns easily are at increased risk. Family history is another risk factor of melanoma. The risk is higher if one or more of the person’s first-degree relatives has had melanoma. The researchers within Chaperon project have recently published two articles on this topic.